Now, Chlamydia pneumoniae is classically considered Gram-negative, because it can't retain the crystal violet dye used during gram staining.
Additionally, it has an outer lipopolysaccharide membrane which all Gram-negative bacteria also have. However, other Gram-negative bacteria also have a thin peptidoglycan layer under the lipopolysaccharide membrane, whereas Chlamydia pneumoniae doesn’t.
Without it, Chlamydia can’t retain the pink safranin dye used during Gram staining, so they’re not technically Gram-negative either, which is why they’re more correctly called atypical bacteria.
To visualize them, a Giemsa stain is required, which makes them look blue or reddish blue.
Now, Chlamydia pneumoniae is non-motile and round-shaped, and is also an obligate aerobe, meaning that they depend on oxygen for survival.
Additionally, Chlamydia pneumoniae are obligate intracellular pathogens which means they are unable to generate ATP so they rely on other cells for energy.
So, this means that they can’t grow on artificial mediums but rather, require a host cell for culture.
Ok now, when C. pneumoniae enters a host cell, it undergoes a life cycle that alternates between two distinct forms.
The first is the small spore-looking form called the elementary body, and it’s the infective form of this bacteria.
On a side note, if this sounds similar to mitosis… well, it is!
But the term binary fission is used to describe division of prokaryotic cells, which don’t have a nucleus, and therefore some steps in replication are different from mitosis.
Now, eventually, binary fission results in a huge number of reticulate bodies, which then start transitioning back to elementary bodies.
So the cell becomes too small, bursting open, and letting out a lot of elementary bodies in the surrounding fluids, where they attach to other cells to repeat the cycle over and over.
Once in the lungs, the elementary body is taken up by epithelial lung cells inside an endosome, through phagocytosis.