DNA structure


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DNA structure

Molecular biology

Molecular biology

Nuclear structure

DNA structure

Transcription of DNA

Translation of mRNA

Gene regulation


Amino acids and protein folding

Protein structure and synthesis

Nucleotide metabolism

DNA replication

Lac operon

DNA damage and repair

Cell cycle

Mitosis and meiosis

DNA mutations

Disorders of molecular biology

Lesch-Nyhan syndrome

Orotic aciduria

Adenosine deaminase deficiency

Xeroderma pigmentosum

Li-Fraumeni syndrome

Bloom syndrome

Fanconi anemia

McCune-Albright syndrome

Acute radiation syndrome

Purine and pyrimidine synthesis and metabolism disorders: Pathology review


DNA structure


0 / 7 complete

USMLE® Step 1 questions

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High Yield Notes

15 pages


DNA structure

of complete


USMLE® Step 1 style questions USMLE

of complete

A researcher is studying the thermal properties of small sequences of deoxyribonucleic acid found in Gram-positive bacteria. Which of the following sequences of DNA will have the highest melting point?  

External References

First Aid







cloning methods p. 53

free radical effect on p. 213

introns vs exons p. 41

laddering in apoptosis p. 206

methylation of p. 32

mutations in p. 38

repair of p. 37

replication of p. 36

DNA topoisomerases p. 36

Mutations in DNA p. 38

Purines p. 191

mutations in DNA p. 36


mutations in DNA p. 36

External Links


Content Reviewers

Rishi Desai, MD, MPH


Tanner Marshall, MS

Buried deep within the nucleus, lies our genetic information, called DNA - which stands for deoxyribonucleic acid.

DNA is made up of two strands that are coiled around one another in a double helix.

Each of the two strands that make up DNA is a polynucleotide chain - so it’s a string of nucleotides one after another.

Nucleotides are organic molecules that are made up of a 5-carbon sugar, a phosphate group and a nitrogenous base - also called a nucleobase - or, simply, a “base”.

For DNA, the 5-carbon sugar is deoxyribose. Deoxyribose looks like a pentagon, and the tips of the pentagon are 4 carbons and an oxygen molecule.

The 5th carbon is outside the ring, and it binds to the phosphate group.

The sugar and phosphate elements are the same for the 4 nucleotides that make up DNA - the difference comes from the nucleobase, which is attached to the first carbon of the sugar.

There are four nucleobases that make up and give DNA nucleotides their name - adenine, or A, thymine, or T, cytosine, or C and guanine, or G.

Structurally, these bases can be either purines or pyrimidines - the purines, guanine and adenine, are made up of 2 heterocyclic rings.

The pyrimidines, cytosine and thymine, are made up of a single ring.

You can remember this with “CUT PYe (pie)” - because cytosine and thymine along with uracil, which is a nucleotide found in RNA, are all Pyrimidines.

The nucleotides bind to one another using their sugar and phosphate groups.

The phosphate group on the 5th carbon of the sugar of one nucleotide - also called the 5’ carbon - forms a covalent bond with the 3rd carbon on the sugar of the next nucleotide - also called the 3’ carbon.

This gives each DNA strand a sugar-phosphate backbone, as well as a “direction” - one of the strands runs from the 5’ end towards the 3’ end, while the other one runs from 3’ to 5’.

This makes DNA an “antiparallel” molecule - it’s a bit like two snakes coiled up together but facing different directions.

However, to form the double helix, the nucleotides use their bases - A, T, C, G to form hydrogen bonds with bases on the opposing strand.

Bases form bonds according to the rule of “complementary base pairing” - which states that in DNA, A always pairs with T, by means of two hydrogen bonds, while C always pairs with G, through three hydrogen bonds.

The hydrogen bonds are much weaker than the covalent bonds that hold the strands together - so they can be easily broken and reformed when DNA is being transcribed into RNA or being replicated during cell division.


The basic structure of DNA is a double helix, which consists of two long strands of DNA wrapped around each other. Each strand consists of many nucleotides, each consisting of a sugar and phosphate group, which form the sugar-phosphate backbone of DNA, and a nitrogenous base, which determines the identity of each nucleotide. The order of the bases (A, T, C, and G) on each strand determines the DNA sequence. DNA strands are held together by hydrogen bonds between the bases.


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