Enterococcus

With Enterococcus, entero- refers to the intestines, while -coccus means round shape.

So Enterococcus is a genus of round bacteria that commonly colonizes the gut of humans and animals.

Enterococcus is also called Group D streptococcus in Lancefield classification developed by an American microbiologist Rebecca Lancefield.

There are two species that can cause infections in humans and these are Enterococcus faecalis, amounting for the majority of infections, and Enterococcus faecium, which causes disease more rarely.

Now, looking at an individual bacterium, Enterococcus has a thick peptidoglycan cell wall, which takes in purple dye when Gram stained - so this is a gram-positive bacteria.

When there’s more of them, Enterococci grow in short chains, usually in pairs.

They’re non-spore forming, facultative anaerobes, meaning that they can survive in both aerobic and anaerobic environments and catalase negative, which means they don’t produce an enzyme called catalase.

Enterococci also can tolerate extreme environmental conditions including high sodium chloride concentrations, high pH and even high temperatures.

They can survive at 60 degrees Celsius for up to 30 minutes!

Ok, now, enterococcus is pyrrolidonyl arylamidase positive, because it makes an enzyme called L-pyrrolidonyl arylamidase.

To test for this, a small sample is taken from a suspected bacterial colony, and then inoculated to a disk pad that’s embedded with pyrrolidonyl beta naphthylamide - another joy of a word.

With Enterococcus, pyrrolidonyl arylamidase hydrolyzes pyrrolidonyl beta-naphthylamide to produce beta-naphthylamide.

Try saying that 3 times fast! Finally, another reagent called N-methylamino-cinnamaldehyde is added to the disk, and it reacts with beta-naphthylamide, resulting in a bright red color that confirms Enterococcus is pyrrolidonyl arylamidase positive.

Now, most commonly, Enterococci are gamma hemolytic which means that when cultivated on blood agar they don’t induce hemolysis, so the agar under and around the colony remains unchanged.

But sometimes, they can induce alpha hemolysis, also called partial hemolysis, which means that the agar under the colony turns dark and greenish.

Finally, it can grow on bile salts, which is very useful to differentiate Enterococci from non-enterococcus group D streptococci.

This can be demonstrated with the Bile-Esculin test, that can be done on tubes or plates and uses a medium that contains peptone, beef extract, bile, esculin, ferric citrate and agar.

So, in the presence of bile, Enterococcus can hydrolyze esculin into glucose and esculetin.

Then, esculetin reacts with the ferric ions supplied by ferric citrate and form a black diffusible complex.