Klebsiella pneumoniae typically causes a pneumonia.
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A 36-year-old man comes to the emergency department because of fever, chills, and breathing issues. He recently suffered from strep throat, for which he was hospitalized and prescribed 10 days of antibiotics. He is coughing with significant sputum that looks like red currant jelly. Which of the following is the most likely diagnosis?
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Klebsiella pneumoniae is a Gram-negative rod-shaped bacteria, which belongs to a family of bacteria called the Enterobacteriaceae.
Now, Klebsiella pneumoniae has a thin peptidoglycan layer, so like other Gram-negative bacteria, it stains pink.
And since it’s a bacillus, it looks like a little pink rod under the microscope.
Klebsiella pneumoniae is non-motile, non-spore forming, and facultative anaerobe.
This means it can live even without oxygen, although it grows better in an aerobic environment.
So, it prefers places like lungs,throat, or respiratory airways, as well as ventilators in the ICU where there is an unlimited flow of oxygen.
This can be tested by transferring a pure sample of bacteria from the culture to a sterile tube containing a mixture of “urea agar” broth and phenol red.
Then, the mixture is incubated.
Ammonia then makes the mixture change color from orange-yellow to bright pink.
Finally, Klebsiella pneumoniae grows well on MacConkey agar, which is a medium that contains a pH sensitive dye and lactose.
This medium helps identify whether Gram-negative bacteria are lactose fermenters or not.
This results in the production of the acid that makes the pH sensitive dye turn pink - so their colonies will be pink.
Klebsiella has an abundant polysaccharide capsule which leads to the formation of very mucoid and viscous pink colonies.
Pathogenesis and virulence factors
First, Klebsiella pneumoniae is encapsulated, which means it’s covered by a polysaccharide layer called a capsule.
This capsule is a major virulence factor because of its antiphagocytic ability.
This means that it protects the bacteria against phagocytosis by macrophages and neutrophils, allowing Klebsiella to escape destruction.
On the capsule, there are pili, which are hair-like extensions that help the bacteria attach to host cells.
Underneath the capsule, there’s an outer membrane, which consist of lipopolysaccharides, or LPS.
Now, LPS has the ability to avoid complement-mediated killing by inhibiting the formation of the membrane attack complex and preventing membrane damage and bacterial cell death.
Finally, it needs iron to thrive and replicate, so it produces a siderophore, which is a term used for a group of small, high-affinity, iron chelating compounds that snatch iron from host cells.
Ammonia can then combine with hydrogen to form ammonium, which increases urine PH - so the urine becomes more alkaline.
Alkaline urine promotes the precipitation of phosphate, calcium, and magnesium.
Finally, this leads to urinary stasis, which starts a vicious circle, promoting bacterial multiplication, urinary alkalinization, and the deposition of new layers of struvite.