Pasteurella multocida

Pasteurella multocida is a gram-negative coccobacillus which is involved in a zoonotic infection.

Most often, it causes soft tissue and respiratory infections.

There are 3 subspecies that cause disease in humans: Pasteurella multocida subspecia multocida, Pasteurella multocida subspecia septica and Pasteurella multocida subspecia gallicida.

All of these subspecies are encapsulated, meaning they have a polysaccharide layer called a capsule.

And depending on the capsular antigens found on the capsule, they can be grouped in 5 different serogroups: A, B, D, E and F.

Each of them is involved in a different type of disease and only serogroups A and D cause disease in humans.

So, serotype A causes respiratory infections and serotype D causes soft tissue infections like cellulitis.

Now, Pasteurella multocida has a thin peptidoglycan layer, so it doesn’t retain the crystal violet dye during gram staining.

Instead, like any other gram-negative bacteria, it stains pink with safranin dye.

But, sometimes, it can have a bipolar staining which means that only the poles of the bacteria stain pink, and the rest of it remains unstained, so the bacteria look like safety pins under the microscope.

Alright, now Pasteurella multocida is non-motile, non-spore forming, and facultative anaerobic which means it can survive in both aerobic and anaerobic environments.

It’s oxidase and catalase positive, which means it can produce both these enzymes, and it’s also nitrate reduction positive which means it can produce an enzyme that hydrolyzes nitrate into nitrite.

Finally, Pasteurella multocida grows well at 37ºc on sheep blood agar, which is the preferred culture medium, but it can also grow on chocolate agar, Mueller-Hinton agar or brain heart infusion agar.

The colonies are opaque or grey, and small - they’re only about 1-2 millimeters in diameter each.

Now, Pasteurella multocida has a number of virulence factors, that are like assault weaponry that help it attack and destroy the host cells, and evade the immune system.

First, on its capsule there are fimbriae, which are hair-like extensions that help the bacteria attach to host cells.