Prader-Willi syndrome is a genetic imprinting disorder resulting from gene deletion and presenting with hyperphagia, obesity, intellectual disability, hypotonia, and hypogonadism.
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A 7-year-old boy with short stature and obese body habitus is brought to his pediatrician for an annual check up. His mother states that he has been doing well in school since he began an individualized education plan. However, he continues to gain weight, and she confesses that she might have been overfeeding him because when he was younger, he was a poor feeder and a floppy baby. She has been trying to restrict his diet, unsuccessfully, as her son will guilt his grandmother into feeding him. Physical examination shows the patient has failed to reach developmental milestones for his age, and his hands and feet appear to be small in proportion to his body. In addition the patient shows evidence of cognitive delay. Which of the following is the best diagnostic test to confirm the underlying cause of the patient's obesity?
Content Reviewers:Rishi Desai, MD, MPH
Prader-Willi syndrome is a genetic disorder that, in infancy, causes poor feeding and low muscle tone, and then in childhood, causes overeating, intellectual disability, and low sex hormones starting in childhood.
Prader-Willi syndrome happens when a handful of genes on chromosome 15 aren’t transcribed into messenger RNA and therefore aren’t expressed.
Among these are SNRPN which stands for Small Nuclear Ribonucleoprotein Polypeptide N and a cluster of snoRNAs, which stands for small nucleolar RNAs, and these genes all have protein products that modify other RNAs.
Now, normally, the copies of the genes contributed by the mother, or maternally derived genes, to this region, are silenced, or turned off, and only the genes from dad, or paternally-derived genes, get expressed.
This special genetic process is called imprinting, where only one copy of the gene gets expressed, not both.
And this differs from most genes in the genome, where both the maternal and paternal copies are expressed.
So those maternal copies in this region are imprinted and therefore silenced.
And this silencing of the maternal copies is an epigenetic process.
In the word “epigenetic”, “epi” means outside of, and “genetic” refers to the DNA sequences of A’s, C’s, G’s, and T’s.
So epigenetic silencing of a gene means turning it off while keeping the DNA sequence itself the same.
The Prader-Willi genes get turned off when methyl groups get attached to the DNA, a process that happens way back when the mother was making an egg.
Even after fertilization of the egg and all of the cell divisions it takes to make a person, that epigenetic mark remains, kind of like a reminder to keep those maternally-derived copies of the genes turned off.
Unfortunately, though, this means that if paternal copies of the genes don’t get expressed, then there aren’t any backup copies being expressed, and so no copies get expressed!
And this is what happens in Prader-Willi syndrome!
Now, there are a few ways these paternal genes wouldn’t be expressed.
The most common one is a deletion on the paternal genes spanning Prader-Willi region.
A lot of deletions also include a nearby gene called OCA2, which codes for a pigment that gives color to the eyes, the hair, and the skin.
So the Prader-Willi patients with deletions that encompass OCA2 can have a light complexion.
Now, a second way is called maternal uniparental disomy, which means two chromosomes from one parent—the mother.
If both copies of chromosome 15 were derived from the mother, that means all the prader willi genes are methylated and therefore silenced, since there aren’t any unsilenced paternal genes.
This can happen when the primary oocyte in the female, which eventually becomes an egg cell, undergoes nondisjunction during meiosis I, which ultimately results in an egg cell with two maternal chromosomes, and after combining with a sperm cell, you get a zygote with three copies—called trisomy 15.
Since trisomy 15 isn’t compatible with life, the fetus only survives only if they lose one copy of the chromosome in the early embryo, called trisomy rescue.
So if it’s the paternal chromosome 15 that’s lost, the result is maternal uniparental disomy 15.
Alright, a third way to get Prader-Willi syndrome, is a mutation in the imprinting center, which is a sequence of DNA near the Prader-Willi genes that directs imprinting via sex-specific methylation.