Blood Components and Function Notes

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Osmosis High-Yield Notes

This Osmosis High-Yield Note provides an overview of Blood Components and Function essentials. All Osmosis Notes are clearly laid-out and contain striking images, tables, and diagrams to help visual learners understand complex topics quickly and efficiently. Find more information about Blood Components and Function:

Blood components

Platelet plug formation (primary hemostasis)

Role of Vitamin K in coagulation

Coagulation (secondary hemostasis)

Clot retraction and fibrinolysis

Blood groups and transfusions

NOTES NOTES BLOOD COMPONENTS & FUNCTION BLOOD COMPONENTS osms.it/blood-components BLOOD COMPONENT SEPARATION ▪ Blood components separate by density in centrifuge ▫ Heaviest layer: erythrocytes ▫ Middle layer: buffy coat ▫ Lightest layer: plasma ERYTHROCYTES ▪ Comprise 45% (hematocrit) of total blood volume ▪ Carry O2 to tissues; bring CO2 to lungs ▪ Biconcave discs (depressed center) ▫ Fit through vessels, ↑ surface area (for gas exchange) ▪ No organelles ▫ ↑ space for hemoglobins BUFFY COAT ▪ Comprises < 1% of total blood volume ▪ Contains platelets, leukocytes ▪ Platelets clump together → seal damaged blood vessels ▪ Leukocytes ward off pathogens, destroy cancer cells, neutralize toxins PLASMA ▪ Comprises 55% of total blood volume ▪ No cells: 90% water + proteins, electrolytes, gases ▪ Albumin: maintains oncotic pressure, acts as transport protein ▪ Globulins: antibodies, transport proteins ▪ Fibrinogen: involved in clot formation (helps platelets attach) ▪ Electrolytes: include sodium, potassium, calcium, chloride, carbonate Figure 43.1 Blood components and their relative proportions. 364 OSMOSIS.ORG
Chapter 43 Hematology: Blood Components & Function PLATELET PLUG FORMATION (PRIMARY HEMOSTASIS) osms.it/platelet-plug-formation-primary-hemostasis ▪ Hemostasis: blood-loss prevention ▪ First two hemostasis steps: platelets clump, form plug around injury site in five steps PLATELET PLUG FORMATION STEPS 1. Endothelial injury ▪ Nerves, smooth muscle cells detect injury ▪ Trigger reflexive contraction of vessel (vascular spasm) → ↓ blood flow, loss ▪ Secretion of nitric oxide, prostaglandins stop; secretion of endothelin begins → further contraction 3. Adhesion ▪ GP1B surface proteins on platelets bind to Von Willebrand factor 4. Activation ▪ Platelet changes shape (forms arms to grab other platelets), releases more von Willebrand factor, serotonin, calcium, ADP, thromboxane A2 (positive feedback loop) ▪ ADP, thromboxane A2 result in GPIIB/IIIA expression 5. Aggregation ▪ GPIIB/IIIA binds to fibrinogen, links platelets → platelet plug 2. Exposure ▪ Damage to endothelial cells exposes collagen ▪ Damaged cells release Von Willebrand factor (binds to collagen) Figure 43.2 Layers of an arterial wall. OSMOSIS.ORG 365
Figure 43.3 Platelet plug formation steps. 366 OSMOSIS.ORG
Chapter 43 Hematology: Blood Components & Function COAGULATION (SECONDARY HEMOSTASIS) osms.it/coagulation-secondary-hemostasis ▪ Last two hemostasis steps: clotting factors activate fibrin, build fibrin mesh around platelet plug ▪ Begins with either extrinsic/intrinsic pathway; factor X activation → coagulation cascade (common pathway) EXTRINSIC PATHWAY 1.Trauma damages blood vessel, exposes cells under endothelial layer ▫ Tissue factor (factor III) embedded in membrane 2.Factor VII in blood binds to tissue factor, calcium → VIIa-TF complex INTRINSIC PATHWAY 1.Circulating factor XII contacts negatively charged phosphates on platelets/ subendothelial collagen → factor XIIa 2.Factor XIIa cleaves factor XI → factor XIa 3.Factor XIa + calcium cleaves factor IX → factor IXa 4. Factor IXa + factor VIIIa (binds to Von Willebrand factor) + calcium → enter the common pathway COMMON PATHWAY 1. Factor X is cleaved → factor Xa 2. Factor Xa cleaves factor V → factor Va 3. Factor Xa + factor Va + calcium → prothrombinase complex ▫ Prothrombin (factor II) → thrombin (factor IIa) 4. Thrombin activates platelets, cofactors (V, VIII, IX); cleaves fibrinogen, stabilizing factor (→ factor XIIIa + calcium → cross-links in mesh) COAGULATION TESTS ▪ Prothrombin time (PT): tests extrinsic pathway ▪ Activated partial thromboplastin time (aPTT): tests intrinsic pathway ROLE OF VITAMIN K IN COAGULATION osms.it/vitamin-k-in-coagulation ▪ Vitamin K regulates blood coagulation ▫ Converts coagulation factors into mature forms ▪ 12 coagulation factors: (I–XIII, no factor VI); factors II, VII, IX, X require vitamin K ▪ Quinone reductase reduces vitamin K quinone (dietary form) into vitamin K hydroquinone ▪ Vitamin K hydroquinone donates electrons to γ-glutamyl carboxylase, converting non-functional forms of II, VII, IX, X into functional forms ▫ Adds chemical group made of one carbon, two hydrogens, one oxygen to glutamic acid residues on proteins ▪ After carboxylation step, vitamin K (as vitamin K epoxide) is converted back into vitamin K quinone via epoxide reductase ▪ Coagulation factors appear in all coagulation pathways OSMOSIS.ORG 367
Figure 43.4 Coagulation steps, including the intrinsic, extrinsic, and common pathways. 368 OSMOSIS.ORG
Chapter 43 Hematology: Blood Components & Function Figure 43.5 Vitamin K cycle. A single molecule of Vitamin K can be reused many times. ANTICOAGULATION, CLOT RETRACTION & FIBRINOLYSIS osms.it/clot-retraction-and-fibrinolysis ANTICOAGULATION ▪ Occurs during primary, secondary hemostasis; regulates clot formation ▪ Prevents clots from growing too large → block blood flow, form emboli ▪ Regulation starts with thrombin (factor II) ▫ Multiple pro-coagulative functions ▫ Proteins C, S bind thrombomodulinthrombin → cleaves, inactivates factors V, VIII ▫ Antithrombin III binds thrombin/factor X → inactivates both (plus factors VII, IX, XI, XII with lower affinity) ▪ Other factors prevent platelets adhering during primary hemostasis ▫ Nitric oxide, prostacyclin → ↓ thromboxane A2 Figure 43.6 Proteins involved in anticoagulation. Thrombomodulin is found on the surface of intact epithelial cells lining blood vessels. OSMOSIS.ORG 369
CLOT RETRACTION ▪ Occurs one hour after primary, secondary hemostasis ▫ Contracts clot ▪ Platelets in clot express integrin αIIBβ3 → binds to fibrin expressing actin, myosin → lamellipodia contract, fibrin mesh tightens closing wood FIBRINOLYSIS ▪ Occurs two days after primary, secondary hemostasis; degrades clot ▪ Plasminogen → plasmin (via tissue plasminogen activator) ▪ Plasmin proteases fibrin → clot dissolves BLOOD GROUPS & TRANSFUSIONS osms.it/blood-groups-and-transfusions BLOOD TRANSFUSIONS ▪ Immune system produces antibodies against absent glycoproteins ▪ Type AB: no antibodies → universal recipients ▪ Type O: no antigens → universal donors ▪ Blood transfusion: person receives blood/ elements of blood (usually through intravenous infusion) ▫ Homologous transfusion: anonymous donor ▫ Autologous transfusion: self-donor (e.g. in planned surgery) ▪ Blood is mixed with calcium oxalate to prevent coagulation, refrigerated/frozen for storage Rh system ▪ Determined by presence of Rh protein ▫ Rh positive; Rh negative ▪ Rh+ can receive blood from either group ▪ Rh- can only receive Rh- blood BLOOD TYPING CROSS MATCHING ▪ Transfusion blood types not compatible → autoimmune reaction (hemolytic transfusion reaction) ▪ Two classification systems (based on presence/absence of proteins) ▫ ABO system ▫ Rh system ABO system ▪ Determined by type of glycoproteins found on red blood cells (RBCs) ▫ Type A; type B; type A & B; type O (neither) 370 OSMOSIS.ORG ▪ Test to confirm donor’s blood is safe for recipient ▪ Recipient serum is mixed with donor blood ▫ Agglutination reaction: cannot receive
Chapter 43 Hematology: Blood Components & Function Figure 43.7 Blood types are reported as ABO group and Rh + or -. When both classification systems are combined, there are eight possible blood types: A+, A-, B+, B-, AB+, AB-, O+, O-. OSMOSIS.ORG 371

Osmosis High-Yield Notes

This Osmosis High-Yield Note provides an overview of Blood Components and Function essentials. All Osmosis Notes are clearly laid-out and contain striking images, tables, and diagrams to help visual learners understand complex topics quickly and efficiently. Find more information about Blood Components and Function by visiting the associated Learn Page.