The primary difference between prokaryotic and eukaryotic cells is that only the latter contain a nucleus and other membrane-bound organelles. Prokaryotic and eukaryotic cells make up prokaryotes and eukaryotes, respectively. Prokaryotes are always
unicellular organisms, while eukaryotes are often multicellular organisms. Additionally, eukaryotic cells are more than 10 to 100 times larger than prokaryotic cells and are much more complex.
While eukaryotes store their
DNA inside the nucleus, prokaryotes keep it in the
cytoplasm. Additionally, DNA in eukaryotic cells is organized into double-stranded chromosomes that are condensed by histones. In contrast, prokaryotic cells contain one primary circular chromosome and may also contain plasmids (small rings of DNA). Eukaryotic cells contain a smaller proportion of coding DNA and a higher proportion of noncoding DNA, compared with prokaryotic cells. The ribosome type in eukaryotic cells is 80S, with 40S and 60S subunits, while prokaryotic cells contain 70S
ribosomes with 30S and 50S subunits.
The locomotive structures (i.e., flagellum) also vary between prokaryotic and eukaryotic cells. In eukaryotic cells, flagella are microtubule bundles composed of dynein and a
plasma membrane, powered by ATP to make a bending motion. Prokaryotic locomotive structures are instead composed of repeated flagellin, a hook, and a motor complex attached to the
cellular membrane, and are powered by protons to make a
rotational motion.
The cell types also vary in their mechanisms of cell division. Prokaryotes can undergo binary fission, where one cell duplicates its contents, the
cytoplasmic membrane elongates, and cytokinesis (i.e., division of the cytoplasm) separates the duplicated DNA molecules, and two identical cells emerge. All eukaryotes undergo a similar but more complicated process called
mitosis. In both binary fission and mitosis, parent cells have the exact same number of chromosomes as their daughter cells. However, in sexually reproducing eukaryotic organisms, another type of cell division called
meiosis allows gene reassortment and creates
genetically unique reproductive cells called gametes, or sex cells. Gametes are haploid cells, meaning they have half the number of chromosomes as the parent cells.