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Translation of mRNA

Translation of mRNA


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High Yield Notes
15 pages

Translation of mRNA

15 flashcards

USMLE® Step 1 style questions USMLE

5 questions

In an attempt to understand proteome variations of the human body, a group of researchers decides to synthesize a protein in the lab and compare it to proteins created via in-vivo. mRNA isolated from a cell is translated under laboratory conditions. However, it is found that the same mRNA produces a protein with different properties and structures in-vivo when compared to in-vitro synthesis. Which of the following best explains this finding?  


Content Reviewers:

Rishi Desai, MD, MPH

Deep within the cell’s nucleus, there’s our DNA. DNA is made up of genes, and each gene is basically a specific part of the DNA that codes for a protein.

And genes become proteins in two steps: transcription and translation.

Transcription is the first step in creating a protein, during which a specific gene is “read” and copied on an individual mRNA, or messenger RNA molecule - which is like a blueprint with instructions on what protein to build.

Translation is the second step, and it’s when organelles called ribosomes assemble the protein from amino acids lying around in the cytoplasm.  

Each mRNA has a “direction” - running from the 5’ end towards the 3’ end.

mRNA is a chain of four types of nucleotides - which are the individual “letters” or “building blocks” of mRNA.

Nucleotides are made out of a ribose, which is a 5-carbon sugar, a phosphate, and one of the four nucleobases - guanine, uracil, adenine, and cytosine - or, commonly, G, U, A, C for short.

These nucleotides are the actual information carried within the RNA - each 3 letters is one amino acid.

In total, these four nucleotides can be arranged into a total of 64 combinations.

The freshly made mRNA floats out of the nucleus through a pore, and hooks up with an idle ribosome to begin getting translated into a protein.

Right away, there’s a process called initiation which is where the ribosome grabs the mRNA.

Groups of 3 “letters” are called codons, and each codon codes for one amino acid, or gives a certain signal, such as start or stop.

So, for the ribosome to start, it needs to find a start codon. That is usually a sequence of A U G - which simultaneously codes for amino acid methionine. So most proteins actually begin with methionine!

When the ribosome runs into this, it knows that from here on, every subsequent codon represents one amino acid in the protein.

Now while each codon codes for a single amino acid; each amino acid can be coded for by one or more codons.

For example - while only the U G G codon codes for amino acid tryptophan, as many as six codons code for the amino acid leucine - U U A, U U G, C U U, C U C, C U A, C U G.

Translation continues until the ribosome runs into a stop codon, which is a sequence of U A A, U A G, or U G A. The stop codons do not code for anything else, except for stopping - which is also known as termination.

But mRNA is only a blueprint of which amino acid goes where in order to get a protein.

The actual job of ferrying the amino acids to the ribosome is done by another type of RNA, called transfer RNA, or tRNA.

tRNA is a very short RNA sequence that is specialized for finding a specific type of amino acid within the cytosol, and bringing it to the ribosome.

tRNA only has a 3 “letter” coding sequence, called the anticodon, which is complementary for what’s written in the mRNA - it means that it can briefly bind to the corresponding mRNA codon and deposit the amino acid within the ribosome.

So, the ribosome has 3 sites for tRNA to bind.

They are the aminoacyl site, or A, the peptidyl site, or P, and the exit site, or E.


Translation is the process by which ribosomes in the cell's cytoplasm decode the genetic information encoded in messenger RNA (mRNA) to synthesize proteins. During translation, transfer RNA (tRNA) molecules bring amino acids to the ribosome, where they are linked together in a specific sequence to form a polypeptide chain. The order of amino acids in the polypeptide chain is determined by the sequence of codons in the mRNA molecule. The process of translation is essential for the production of proteins, which are involved in a wide variety of biological processes in the cell.