VDJ rearrangement

00:00 / 00:00


VDJ rearrangement


0 / 5 complete

USMLE® Step 1 questions

0 / 1 complete

High Yield Notes

18 pages


VDJ rearrangement

of complete


USMLE® Step 1 style questions USMLE

of complete

A maturing T cell undergoes VDJ rearrangement. A strand of DNA containing a V and D gene is looped on itself, and a piece of DNA between these two genes is cut out. These two pieces of DNA are subsequently reattached. Which of the following enzymes is responsible for adding random nucleotides to promote further diversity at this stage of T cell formation?  

External References

First Aid








V(D)J recombination p. 97

External Links


Content Reviewers

The adaptive immune response is highly specific for each invader, and that’s because the cells of the adaptive immune response have receptors that differentiate friendly bacteria and potentially deadly pathogens from their unique parts - called antigens.

The key cells of the adaptive immune response are the lymphocytes- the B and T cells.

And the antigen receptors for T cells and B cells have a lot of things in common, one of which is that they share the same mechanism - called VDJ rearrangement - to generate a massively diverse set of receptors.

VDJ stands for variability, diversity, and joining, respectively, and VDJ rearrangement has 4 key characteristics that help ensure that each antigen receptor is unique.

First, each individual inherits multiple V, D, and J gene segments; second, the V, D, and J gene segments randomly recombine - meaning that any V can pair with any D and any J; third, there’s recombinational inaccuracy - meaning that this process is purposefully error prone - which leads to additional variation; and fourth, there’s random reassortment of two chains - meaning that this process involves two different chains that come together to make the receptor.

First, let’s look at our antigen receptors. The B cell receptor, or BCR, is essentially an antibody, except that it has a transmembrane part that goes through the membrane and attaches the receptor to the surface of a B cell.

Just like antibodies, the B cell receptor has a heavy chain and a light chain.

One region or fragment of the B cell receptor binds the antigen and it’s called the fragment - antigen binding or Fab region.

There are two Fab regions on every B cell receptor.

Then there’s the constant region or fragment called the fragment - constant or Fc region, which is the part that determines what class of B cell receptor it will be - for example, IgM or IgD, and whether or not it will remain a membrane bound B cell receptor or if it will get secreted as a free floating antibody.


VDJ rearrangement is a process by which the genes responsible for encoding the variable region of the B-cell receptor and T-cell receptor are rearranged to create a diverse repertoire of receptors capable of recognizing a wide variety of antigens.

During VDJ rearrangement, DNA segments called V (variable), D (diversity), and J (joining) are rearranged to form the coding sequence for the receptor variable region. The process is mediated by recombination-activating genes (RAG1 and RAG2) and involves the introduction of double-strand breaks at specific recombination signal sequences (RSS) flanking the V, D, and J segments, followed by joining of the broken ends.

The resulting receptor variable region diversity is crucial for adaptive immunity, allowing the immune system to recognize and respond to a wide range of potential pathogens. However, errors in VDJ rearrangement can lead to autoimmune disease, immunodeficiency, or cancer.


Copyright © 2023 Elsevier, its licensors, and contributors. All rights are reserved, including those for text and data mining, AI training, and similar technologies.

Cookies are used by this site.

USMLE® is a joint program of the Federation of State Medical Boards (FSMB) and the National Board of Medical Examiners (NBME). COMLEX-USA® is a registered trademark of The National Board of Osteopathic Medical Examiners, Inc. NCLEX-RN® is a registered trademark of the National Council of State Boards of Nursing, Inc. Test names and other trademarks are the property of the respective trademark holders. None of the trademark holders are endorsed by nor affiliated with Osmosis or this website.