Video - ELISA (Enzyme-linked immunosorbent assay)

ELISA is a biochemical assay used in immunology to detect the presence of an antigen, antibody, or another protein. It takes its name from the enzyme-linked immunosorbent assay (ELISA).

The basic principle behind ELISA is that if an antigen or antibody is present in a sample, it will bind to a specific antibody or antigen attached to a solid support. The bound antigen or antibody can then be detected using an enzyme-linked secondary antibody that recognizes the primary antibody or antigen. The presence of this enzyme can be detected using various methods depending on the assay format, such as spectrophotometry, fluorimetry, or chemiluminescence. ELISA is a very sensitive assay that can detect minute levels of antigens or antibodies in a sample. It is often used to measure the concentration of proteins in body fluids, such as serum or urine.