Measuring renal plasma flow and renal blood flow

Definitions & Key takeaways

Renal plasma flow (RPF) is the volume of blood plasma passing through the kidneys per minute; whereas renal blood flow (RBF) is the volume of blood flowing through the renal arteries per minute. Both RPF and RBF are measured in milliliters per minute (ml/min), and both are important measures of kidney function. High values indicate good kidney function, while low values indicate poor kidney function.

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Introduction0:00–0:13

Renal blood flow refers to the amount of blood that the kidneys receive over a period of time. Blood gets to the kidneys through the renal artery.

Glomerulus0:13–1:05

Blood from the renal artery flows into smaller and smaller arteries, eventually forming the tiniest of arterioles called the afferent arterioles.
After the afferent arteriole, blood moves into a tiny capillary bed called the glomerulus. The glomerulus is part of the functional unit of the kidney, called the nephron.
There’s about 1 million nephrons in each kidney, and each of them consists of a renal corpuscle - made up of the glomerulus and the Bowman’s capsule surrounding it - and a renal tubule.
Interestingly, once the blood leaves the glomerulus, it does not enter into venules. Instead the glomerulus funnels blood into efferent arterioles which divide into capillaries a second time.
These capillaries are called peritubular capillaries - because they are arranged around the renal tubule. Now, blood filtration starts in the glomerulus, where an urine precursor called filtrate is formed.

Blood Filtration1:05–1:57

The amount of blood filtered into the nephrons by all of the glomeruli each minute is called the glomerular filtration rate, and it’s actually just a small fraction of the blood that gets to the kidneys, because the glomerulus doesn’t allow red blood cells and proteins to pass through and be excreted into urine.
So right from the start, what passes through the glomerulus is mostly plasma - which normally makes up about 55% of blood.
What is more, the glomerulus only filters about 20% of that plasma in one go. So when all is said and done, of those around 1.25 liters that the heart pumps out every minute, glomerular filtration rate is normally around 125 milliliters.
That plasma-derived filtrate then enters the renal tubule. The renal tubule is made up of a proximal convoluted tubule, the nephron loop - also known as the loop of Henle - which has an ascending and a descending limb - and finally the distal convoluted tubule.

Renal Tubule1:57–2:44

As filtrate makes its way through the renal tubule, waste and molecules such as ions and water are secreted from the peritubular capillaries into the tubule, and they are also absorbed from the tubule back into the capillaries.
The peritubular capillaries reunite to form larger and larger venous vessels. The veins follow the path of the arteries, but in reverse - so they keep uniting until they finally form the large renal vein, which exits the kidney and drains into the inferior vena cava.

True Renal Plasma Flow2:44–5:47

Measuring renal plasma flow is based on the Fick principle - which states that the amount of a substance in the blood that flows into an organ is the amount that must flow out of that organ, assuming that the organ doesn’t produce or degrade the substance.
So what goes in equals what comes out, that’s easy enough. So to measure true renal plasma flow, the amount of plasma that flows into the kidney, we can use para aminohippuric acid - or PAH.
That’s because PAH isn’t made in the body, so a known amount of PAH can be injected into the body. PAH is also ideal because it doesn’t alter renal plasma flow in any way.
So, applying the Fick principle, the amount of PAH entering the kidneys through the renal arteries equals the amount of PAH in the urine plus the amount of PAH leaves the kidneys through the renal veins.
So let’s puts this in the shape of an equation, which uses the concepts of concentration - measured in milligrams per milliliter of fluid - and flow - measured in milliliters per minute.
The amount of PAH entering the kidney per unit time is equal to the concentration of PAH in renal artery blood times renal plasma flow - which is what we want to determine.
The amount of PAH leaving the kidney per unit time is the sum of the concentration of PAH in renal vein blood times renal plasma flow plus the urinary concentration of PAH times the urine flow rate.
Remember that how much PAH enters the kidney is equal to how much PAH leaves the kidney - and this is how we get our equation.
Ok, so to solve this and find out our renal plasma flow, we first take the renal vein concentration of PAH times renal plasma flow and cross it over to the left side of the equation, so the equation becomes: renal artery concentration of PAH times renal plasma flow minus renal vein concentration of PAH times renal plasma flow is equal to urinary concentration of PAH times urine flow.
On the left side, we can take out renal plasma flow, so that side is simply renal plasma flow times the difference in PAH concentration between the renal artery and renal vein.
Finally, to figure out our renal plasma flow, the last step is to divide through by the difference in PAH concentrations.
Thus, renal plasma flow equals the urinary concentration of PAH times urine flow over the difference in PAH concentrations between the renal artery and the renal vein.
So to determine renal plasma flow, we’d have to sample blood from the renal artery, the renal vein, determine the concentration of PAH in both, then determine the flow rate of urine and determine the concentration of PAH in the urine.

Effective Renal Plasma Flow5:47–7:20

While in theory that sounds doable, it’s not easy to obtain blood from the renal artery and vein - and that’s why, in practice, effective renal plasma flow is determined.
The effective renal plasma flow is estimated based on a few key assumptions. First, for most human beings 90% of the PAH that enters the kidneys through the renal artery is excreted into urine, and that the other 10% enters the renal vein.
That means that we can simply ignore the renal vein concentration of PAH, and just assume that the effective renal plasma flow is about 90% of true renal plasma flow.
Secondly, since the kidneys are the only organs that uptake PAH, the PAH concentration in the renal artery is the same as the PAH concentration in any peripheral vein - which makes obtaining blood much easier.
So, the effective renal plasma flow equals the urinary concentration of PAH times urine flow which we can get from testing and monitoring the urine, divided by the plasma concentration of PAH which we can get from a peripheral vein.
Finally, once we’ve measured renal plasma flow, there’s a much simpler formula to calculate renal blood flow. Renal blood flow is equal to renal plasma flow over 1 minus the hematocrit.
The hematocrit refers to the fraction of blood volume occupied by red blood cells, so 1 minus the hematocrit is how much of the blood volume is occupied by plasma.
All right, as a quick recap: measuring renal plasma flow is based on the Fick principle, and uses an agent called para amino hippuric acid - or PAH for short.

Review7:20–8:00

In practice, effective renal plasma flow is determined, and it’s about 90% of true renal plasma flow. Effective renal plasma flow is equal to the clearance of PAH, which can be calculated as the urinary concentration of PAH times urine flow over plasma concentration of PAH - as sampled from a peripheral vein.
After determining renal plasma flow, renal blood flow can be calculated, and it’s equal to renal plasma flow over 1 minus the hematocrit.